Enhancer Detail

Comprehensive information about experimentally validated enhancers

About Enhancer
Enhancer ID: E_04542
Species: Mus musculus
Position : chr5:29314497-29315862
Year: 2022
Title: Cohesin is required for long-range enhancer action at the Shh locus.
Genome Build: mm10
Enhancer Type: Enhancer
Condition: Normal
PMID:  36097291
Tissue: limb bud
Tissue Ontology ID: UBERON:0004347
Cell Source: ES-E14
CVCL ID: CVCL_C320
Cell Type: embryonic stem cell
Cell Ontology ID: CL:0002322
Experiment Type: Synthetic enhancer activation assay; Cohesin/CTCF functional perturbation assay; Chromatin conformation analysis
High Throughput Method: Hi-C data reanalysis; virtual 4C
Low Throughput Method: TALE-VP64 synthetic enhancer activation; auxin-inducible SCC1/RAD21 degradation; auxin-inducible CTCF degradation; RNA FISH; DNA FISH; immunofluorescence; flow cytometry
About Target Gene
Target gene : Shh
About TF
TF name : NA
About Regulatory State
Regulatory State : Active
Upstream Pathway Annotation of TF
GeneName Pathway Name Source Gene Number
Enhancer Associated Network
Overlapping Enhancers (mm10) 4 found
Enhancer ID Position Tissue Cell Disease
E_04039 chr5:29314591-29316266 limb bud limb mesenchyme cell NA
E_00900 chr5:29314881-29315667 limb bud mesenchymal cell NA
E_00901 chr5:29314881-29315667 limb bud mesenchymal cell NA
E_00902 chr5:29314881-29315667 limb bud mesenchymal cell NA
Expression of Target Genes for the Enhancer