About Enhancer
| Enhancer ID: | E_04549 |
| Species: | Homo sapiens |
| Position : | chr14:22556439-22556536   |
| Year: | 2025 |
| Title: | A pooled CRISPR screen identifies the T汐2 enhancer element as a driver of TRA expression in a subset of mature human T lymphocytes. |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 40160815 |
| Tissue: | blood |
| Tissue Ontology ID: | UBERON:0000178 |
| Cell Source: | Jurkat E6.1 |
| CVCL ID: | CVCL_0367 |
| Cell Type: | T cell |
| Cell Ontology ID: | CL:0000084 |
| Experiment Type: | CRISPR-based enhancer screening assay; Functional enhancer perturbation assay |
| High Throughput Method: | Pooled tiled CRISPR/Cas9 screen; sgRNA amplicon Illumina deep sequencing |
| Low Throughput Method: | Targeted CRISPR/Cas9 editing of T汐2; Paired-sgRNA deletion; Flow cytometry; Single-cell cloning; PCR; Allele-specific Sanger sequencing |
About Target Gene
| Target gene : | TRAC |
About TF
| TF name : | NA |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer Associated Network
Overlapping Enhancers (hg38)
1 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_04531 | chr14:22556439-22556536 | blood | T cell | NA |
Expression of Target Genes for the Enhancer