About Enhancer
| Enhancer ID: | E_04574 |
| Species: | Homo sapiens |
| Position : | chr12:48369059-48369213   |
| Year: | 2013 |
| Title: | Tensile strain increases expression of CCN2 and COL2A1 by activating TGF-ζ±-Smad2/3 pathway in chondrocytic cells. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 23631855 |
| Tissue: | cartilage tissue |
| Tissue Ontology ID: | UBERON:0002418 |
| Cell Source: | SW1353 |
| CVCL ID: | CVCL_0543 |
| Cell Type: | chondrocyte |
| Cell Ontology ID: | CL:0000138 |
| Experiment Type: | Enhancer occupancy assay; functional response assay; Enhancer activity assay; TF binding assay |
| Low Throughput Method: | COL2A1 enhancer ChIP-qPCR; co-immunoprecipitation; RT-qPCR; Western blot; cyclic tensile strain assay; Luciferase reporter assay; ChIP; Reporter assay |
About Target Gene
| Target gene : | COL2A1 |
About TF
| TF name : | SOX9 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| SOX9 | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| SOX9 | TGF_beta_Receptor | netpath | 220 |
| SOX9 | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
| SOX9 | Hs_Spinal_Cord_Injury_WP2431_87678 | wikipathways | 21 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
17 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01553 | chr12:48368875-48369590 | cartilage tissue | chondrocyte | NA |
| E_01554 | chr12:48368875-48369590 | cartilage tissue | chondrocyte | NA |
| E_01555 | chr12:48368875-48369590 | cartilage tissue | chondrocyte | NA |
| E_01556 | chr12:48368875-48369590 | cartilage tissue | chondrocyte | NA |
| E_01566 | chr12:48368875-48369590 | cartilage tissue | chondrocyte | osteoarthritis |
| E_01567 | chr12:48368875-48369590 | cartilage tissue | chondrocyte | osteoarthritis |
| E_01568 | chr12:48368875-48369590 | cartilage tissue | chondrocyte | osteoarthritis |
| E_01569 | chr12:48368875-48369590 | cartilage tissue | chondrocyte | osteoarthritis |
| E_01585 | chr12:48368875-48369590 | breast | NA | osteoarthritis |
| E_01586 | chr12:48368875-48369590 | breast | NA | osteoarthritis |
| E_01587 | chr12:48368875-48369590 | breast | NA | osteoarthritis |
| E_01588 | chr12:48368875-48369590 | breast | NA | osteoarthritis |
| E_05292 | chr12:48368959-48369159 | NA | malignant cell | NA |
| E_05293 | chr12:48368959-48369159 | NA | malignant cell | NA |
| E_05298 | chr12:48368959-48369159 | NA | NA | NA |
| E_05299 | chr12:48368959-48369159 | NA | NA | NA |
| E_04571 | chr12:48369059-48369213 | NA | NA | NA |
Expression of Target Genes for the Enhancer